Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation


Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Malysheva SV, Wunderlich S, Haase A, Göhring G, Martin U, Merkert S
Journal Stem cell research
Publication Date 2018 Jul;30:117-121
PubMed 29852468
DOI 10.1016/j.scr.2018.05.015

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