NS-9

General#

Cell Line

hPSCreg Name IMHe012-A
Alternative name(s)
NS-9
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator ISTANBUL MEMORIAL HOSPITAL (IMH)
Derivation country Turkey

External Databases

Cellosaurus CVCL_C806

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases
Disease associated phenotypes
  • chromosomal abnormalities

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2005-05-13
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 4
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 15 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)