Generation of CD16A gene knockout human embryonic stem cell line using CRISPR/Cas9


CD16A is a receptor for the Fc portion of immunoglobulin G, and is involved in the antibody dependent cellular cytotoxicity (ADCC) of nature killer cells(Zhu et al., 2020) and antibody dependent enhancement (ADE) of virus infections(Wan et al., 2020). However, the role of CD16A in human embryonic stem cell modeled development has been merely documented. Hence, to illustrate the role of CD16A in the human cell development, we reported a CD16A knockout human embryonic stem(hESC) cell line via CRISPR/Cas9 mediated gene knockout. The CD16A mutated cell line displayed normal karyotype, pluripotent stem cell marker gene expression and differentiation potential. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wu S, Deng H, Lu B, Yuan L, Wang X
Journal Stem cell research
Publication Date 2022 Oct;64:102935
PubMed 36219983
DOI 10.1016/j.scr.2022.102935

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