Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene


Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Khudiakov A, Kostina D, Zlotina A, Nikulina T, Sergushichev A, Gudkova A, Tomilin A, Malashicheva A, Kostareva A
Journal Stem cell research
Publication Date 2017 Oct;24:77-80
PubMed 29034897
DOI 10.1016/j.scr.2017.08.015

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