CBNK-iPSC

The cell line is not validated yet.

General

Cell Line

hPSCreg name MUSIi013-A
Cite as:
MUSIi013-A (RRID:CVCL_A0ZV)
Alternative name(s)
CBNK-iPSC
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MUSIi013-A-2
(CAR-TIM3 iPSCs)
Last update 18th October 2023
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Provider

Generator Faculty of Medicine Siriraj Hospital (MUSI)

External Databases

Cellosaurus CVCL_A0ZV
Wikidata Q108820996
BioSamples SAMEA114511201

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA114502804

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Mrs. Kanpitchar Tangkiettrakul
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Natural killer cells are cells that resemble large granular lymphocytes. They do not express markers of either T or B cell lineage. They are positive for CD16, CD56, and CD 94. These cells do possess Fc receptors for IgG and can kill target cells using antibody-dependent cell-mediated cytotoxicity. They can also use perforin to kill cells in the absence of antibody and killing may occur without previous sensitization.
Synonyms
  • NK Cell
  • NK-LGL
  • killer cell
  • NK Cells
  • NK cell
  • Killer Cells
  • NK-Cell
  • Killer Cell
  • Natural Killer Cell
  • natural killer cell
  • NK
  • NATURAL KILLER CELL
show more synonyms
Source cell origin
Blood present in the umbilical vessels at the time of delivery. If cryopreserved at birth, cord blood can serve as a source of hematopoietic progenitor cells for transplantation to a patient later diagnosed and treated for a hematopoietic disorder.
Synonyms
  • Cord Blood
  • Umbilical Cord Blood
  • CORD BLOOD
  • umbilical cord blood
  • Unmanipulated Cord Blood
  • Cord blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Other medium:
Base medium: NutriStem
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Morphology pictures
Phase-contrast microphotograph of CBNK-iPSCs clone #5
1) Use Flow cytometric analysis of surface expression of SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 2) Use immunofluorescent staining to determine nuclear expression of OCT4, NANOG and SOX2 proteins 3) Use RT-qPCR analysis to quantify the expression of OCT4, NANOG and SOX2 mRNA compared to H1-ES cells
Method documentation
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro spontaneous differentiation
Morphology
AFP staining
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro spontaneous differentiation
Morphology
alpha-SMA staining
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Morphology
Nestin staining

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)