Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A


Natural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripotent stem cell (iPSC) line highly expressed pluripotency markers, had the capacity to differentiate into derivatives of the three germ layers, while retained a normal karyotype. This cell line may be a useful tool to study epigenic memory that may predispose hiPSCs to enhanced NK differentiation. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Klaihmon P, Luanpitpong S, Lorthongpanich C, Laowtammathron C, Waeteekul S, Terbto P, Issaragrisil S
Journal Stem cell research
Publication Date 2021 Aug;55:102472
PubMed 34311438
DOI 10.1016/j.scr.2021.102472

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