Generation of a heterozygous GAPDH-Luciferase human ESC line (HVRDe008-A-1) for in vivo monitoring of stem cells and their differentiated progeny


Human stem cell-derived beta (SC-β) cells are a candidate for cell replacement therapy for type 1 diabetes. Whilst refinements to the differentiation protocol have resulted in the production of SC-β cells that resemble adult beta cells, the unsolved challenge to protect transplanted SC-β cells from the host immune system remains. To monitor the survival of SC-β cells in vivo, we knocked-in the Firefly luciferase gene into the GAPDH locus of the HUES8 human embryonic stem cell (hESC) line, such that differentiated islet cells constitutively express luciferase. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Gerace D, Boulanger KR, Hyoje-Ryu Kenty J, Melton DA
Journal Stem cell research
Publication Date 2021 May;53:102371
PubMed 34088000
DOI 10.1016/j.scr.2021.102371

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