Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells
Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies. Copyright © 2015 Elsevier B.V. All rights reserved.
|Authors||Montes R, Romero T, Cabrera S, Ayllon V, Lopez-Escamez JA, Ramos-Mejia V, Real PJ|
|Journal||Stem cell research|
|Publication Date||2015 Nov;15(3):614-7|