Establishment of PLAFMCi007-A, an induced pluripotent stem cell line, from peripheral blood mononuclear cells (PBMCs) of a healthy adult woman
Summary
Induced pluripotent stem cell (iPSC) lines for studies investigating many diseases can be established from peripheral blood mononuclear cells; here, an iPSC line was established from CD34+ cells isolated from the peripheral blood of a healthy woman. The cells were electrotransfected with three different recombinant plasmids to generate a normal-karyotype iPSC line that expresses characteristic surface markers and other pluripotent stem cell genes and can differentiate into all three germ layers in vivo. These newly established iPSC lines, a normal human cell line, can serve as a control line in studies investigating the pathogenesis of various diseases and meet the conditions for organoid studies. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Shi M, Shang S, Yang Y, Li Q, Bai XY |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 May;61:102760 |
| PubMed | 35339884 |
| DOI | 10.1016/j.scr.2022.102760 |