An integrated biomanufacturing platform for the large-scale expansion and neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells
Summary
In this work, we developed a microcarrier (MC)-based culture system that allows for the expansion and neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells (hNPCs) under defined conditions. In turn, this MC approach was implemented in a rotating wall vessel (RWV) bioreactor for the large-scale expansion and neuronal differentiation of hNPCs. This work is of significance as it overcomes current limitations of conventional two dimensional (2-D) culture systems to enable the generation of hNPCs and their neuronal derivatives in quantities required for downstream applications in disease modeling, drug screening, and regenerative medicine. Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
| Authors | Srinivasan G, Morgan D, Varun D, Brookhouser N, Brafman DA |
|---|---|
| Journal | Acta biomaterialia |
| Publication Date | 2018 Jul 1;74:168-179 |
| PubMed | 29775730 |
| PubMed Central | PMC8171278 |
| DOI | 10.1016/j.actbio.2018.05.008 |