CBiPS 4F 10b
ESi041-C
General#
Cell Line |
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hPSCreg Name | ESi041-C |
Alternative name(s) |
CBiPS 4F 10b
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors | |
Derivation country | Spain |
External Databases |
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Cellosaurus | CVCL_9S24 |
General Information |
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Publications | |
Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unkown
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Donor Relations |
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Other cell lines of this donor |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell origin |
umbilical cord bloodblood that remains in the placenta and in the attached umbilical cord after childbirth[WP].
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Source cell type (free text) | CD133+ |
Age of donor (at collection) | fetal |
Passage number reprogrammed | 1 |
Reprogramming method |
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Vector type | Integrating |
Vector free reprogramming |
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Other |
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Selection criteria for clones | morphological |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Gelatin | ||||||||||||||||||
Feeder cells |
human foreskin fibroblats Cellfinder Ont Id: CELDA_000001419 |
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Passage method | Mechanically | ||||||||||||||||||
O2 Concentration | 21 % | ||||||||||||||||||
CO2 Concentration | 5 % | ||||||||||||||||||
Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
ASMA |
Yes |
ASA |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
Tuj1 |
Yes |
GFAP |
Yes |
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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