Generation of 5 induced pluripotent stem cell lines, LUMCi007-A and B and LUMCi008-A, B and C, from 2 patients with Huntington disease
Summary
Huntington disease (HD) is an autosomal dominant, neurodegenerative disease caused by a CAG repeat expansion within the coding sequence of the HTT gene, resulting in a highly toxic protein with an expanded polyglutamine stretch that forms typical protein aggregates throughout the brain. We generated human induced pluripotent stem cells (hiPSCs) from two HD patients using non-integrating Sendai virus (SeV). The hiPSCs display a normal karyotype, express all pluripotency markers, have the same CAG repeat expansion as the original fibroblasts and are able to differentiate into the three germ layers in vitro. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | van der Graaf LM, Gardiner SL, Tok M, Brands T, Boogaard MW, Pepers BA, Eussen B, de Klein A, Aziz NA, Freund C, Buijsen RAM, van Roon-Mom WMC |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Aug;39:101498 |
| PubMed | 31326748 |
| DOI | 10.1016/j.scr.2019.101498 |