Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
Summary
SCN5A gene loss-of-function mutations are commonly associated with Brugada syndrome, which represents a risk of lethal arrhythmias and sudden cardiac death. The present report describes the generation of two human induced pluripotent stem cell (iPSC) lines reprogrammed from two Brugada syndrome affected patients carrying SCN5A mutations, c.53506 G>A and c.2102 C>T, respectively. Pluripotency markers, karyotype stability, and differentiation capability into derivatives of the three germ layers were assessed and described in the present report. These lines can be used as a reliable cell model for Brugada syndrome investigations and characterization of leading cellular mechanisms. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Belbachir N, Lai C, Rhee JW, Zhuge Y, Perez MV, Sallam K, Wu JC |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Dec;57:102605 |
| PubMed | 34856468 |
| DOI | 10.1016/j.scr.2021.102605 |