MEL-2

General#

Cell Line

hPSCreg Name SCSe002-A
Alternative name(s)
MEL-2
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Stem Cell Sciences plc (SCS)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_B864
NIHhESC NIHhESC-11-0140

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent

hESC Derivation#

Embryo stage Morula
ZP removal technique Enzymatic
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum
Serum concentration: %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-81
Yes

Microbiology / Virus Screening

Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)