MEL-2
SCSe002-A
General#
Cell Line |
|
| hPSCreg Name | SCSe002-A |
| Alternative name(s) |
MEL-2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Stem Cell Sciences plc (SCS)
Contact:
Embryonic Stem (ES) Cell Biology |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_B864 |
| NIHhESC | NIHhESC-11-0140 |
| CLO | CLO_0100184 |
| Wikidata | Q54952665 |
General Information |
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| Publications |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics#
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-11-0140 |
hESC Derivation#
| Embryo stage | Morula |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum Serum concentration: % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
|
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
|
Microbiology / Virus Screening |
|
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 5
|
Other Genotyping (Cell Line) |
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