SCVI2535

General

Cell Line

hPSCreg name SCVIi081-A
Cite as:
SCVIi081-A (RRID:CVCL_D0L1)
Alternative name(s)
SCVI2535
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SCVIi084-A
(SCVI2591)
Donor diseases:
breast cancer
SCVIi082-A
(SCVI2838)
Donor diseases:
breast cancer
SCVIi083-A
(SCVI2655)
Donor diseases:
breast cancer
UKAi002-A
(PBMNC2_hiPS_007, PV1 JAK2)
Donor's gene variants:
JAK2, JAK2
Donor diseases:
Polycythemia Vera
UKAi002-B
(PBMNC2_hiPS_009, PV1 JAK2 V617F het)
Donor's gene variants:
JAK2, JAK2
Donor diseases:
Polycythemia Vera
TISSUi005-A
(HUMIMIC105, StemUse105, SU105)
Donor diseases:
Leukemia
Last update 16th August 2023
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Provider

Generator Stanford Cardiovascular Institute (SCVI)
Owner Stanford Cardiovascular Institute (SCVI)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA113429116
Cellosaurus CVCL_D0L1
Wikidata Q123033421

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Cell line can only be used in: research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • breast tumor
  • breast tumour
  • mammary tumor
  • mammary tumour
  • breast cancer
  • cancer of breast
  • malignant breast neoplasm
  • malignant breast tumor
  • malignant breast tumour
  • malignant neoplasm of breast
  • malignant neoplasm of the breast
  • malignant tumor of breast
  • malignant tumor of the breast
  • malignant tumour of breast
  • malignant tumour of the breast
  • mammary cancer
  • primary breast cancer
  • mammary neoplasm
show more synonyms
Genetic variants
BRCA2: 13q13.1
NM_000059.4(BRCA2):c.8167G>C (p.Asp2723His)
NP_000050.3:p.Asp2723His

External Databases (Donor)

BioSamples SAMEA113429117

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Joseph C. Wu
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information https://med.stanford.edu/scvibiobank/people.html
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Administrative Panel of Human Subjects Research
Approval number 29904
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Morphology
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
TBX6
Yes
Morphology
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes
PAX6
Yes
Morphology
Protocol or reference

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal chromosome profiles

Other Genotyping (Cell Line)