SCVI2838
SCVIi082-A
General
Cell Line |
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| hPSCreg name | SCVIi082-A |
| Cite as: | SCVIi082-A (RRID:CVCL_D0L2) |
| Alternative name(s) |
SCVI2838
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UKAi002-A (PBMNC2_hiPS_007, PV1 JAK2) Donor's gene variants: JAK2, JAK2 Donor diseases: Polycythemia Vera UKAi002-B (PBMNC2_hiPS_009, PV1 JAK2 V617F het) Donor's gene variants: JAK2, JAK2 Donor diseases: Polycythemia Vera |
| Last update | 16th August 2023 |
| User feedback | |
Provider |
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| Generator | Stanford Cardiovascular Institute (SCVI) |
| Owner | Stanford Cardiovascular Institute (SCVI) |
External Databases |
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| BioSamples | SAMEA113436892 |
| Cellosaurus | CVCL_D0L2 |
| Wikidata | Q123033423 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Cell line can only be used in: research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA113436916 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | Joseph C. Wu |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | https://med.stanford.edu/scvibiobank/people.html |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Administrative Panel of Human Subjects Research |
| Approval number | 29904 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
mTeSR™ Plus
|
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Protocol or reference
In vitro directed differentiation
Protocol or reference
In vitro directed differentiation
Protocol or reference
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
normal
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Other Genotyping (Cell Line) |
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