AD-PS1-ISO1
The cell line is not validated yet.
IRMBi001-A-1
General
Cell Line |
|
| hPSCreg name | IRMBi001-A-1 |
| Cite as: | IRMBi001-A-1 |
| Alternative name(s) |
AD-PS1-ISO1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 29th January 2025 |
| User feedback | |
Provider |
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| Generator | Institute for Neurosciences of Montpellier (INM) |
| Owner | Institute for Neurosciences of Montpellier (INM) |
| Distributors | |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA117637110 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Is the medical history available upon request? | Yes |
| Is clinical information available? | Yes |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
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| BioSamples | SAMEA104618388 |
Ethics
Also have a look at the ethics information for the parental line
IRMBi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene, IdtDNA |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
IRMBi001-A.
|
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| Passage number reprogrammed | 3 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
relesr
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
Morphology pictures
The trilineage differentiation kit (Stem Cell Technologies) was used to assess the pluripotency. It was performed following the manufacturer’s instructions.
Immunostaining ot the 3 lineage markers were performed
Method documentation
Differentiation Potency
In vitro directed differentiation
Morphology
In vitro directed differentiation
Morphology
In vitro directed differentiation
Morphology
Microbiology / Virus Screening |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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