Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background
Summary
A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang Y, Yu H, Zhang Y, Zhang J, Chen K, Sun H, Meng S, Li G, Lei Y, Zhao J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Mar;27:57-60 |
| PubMed | 29328972 |
| DOI | 10.1016/j.scr.2018.01.005 |