HHUUKDi011-A

AATD iPSC-1, ISRM-AATD-iPSC-1

General

Cell Line

hPSCreg Name
HHUUKDi011-A
Alternative name(s)
AATD iPSC-1, ISRM-AATD-iPSC-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 6th April 2022
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Provider

Generator Heinrich-Heine-Universität Düsseldorf (HHUUKD)
Owner Institute for Stem Cell Research and Regenerative Medicine

External Databases

BioSamples SAMEA13944731
CLO CLO_0103831
Cellosaurus CVCL_C1PU

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

With appropriate MTA

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alpha-1 Antitrypsin Deficiency
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • A-1ATD
  • Alpha-1 Antitrypsin
  • Alpha-1-Antitrypsin Deficiency
  • Alpha 1-Antitrypsin Disease
  • Alpha-1 Antitrypsin Deficiency
  • A1AD
show more synonyms

External Databases (Donor)

BioSamples SAMEA13944634

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Prof. Dr. Pavel Strnad, pstrnad@ukaachen.de
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Prof. Dr. Pavel Strnad, pstrnad@ukaachen.de
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethical committee of the medical faculty at Heinrich-Heine university Düsseldorf
Approval number 2021-1627
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
kidney cell
Source cell origin
Urine
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method PBS without Mg2+ and Ca2+
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
Morphology pictures
Morphology of IPSC line
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
SOX17 staining of HHUUKDi011-A
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
aSMA staining of HHUUKDi011-A
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology
TUBB3 staining of HHUUKDi012-A

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Passage number: p9

Other Genotyping (Cell Line)