Induced pluripotent stem cell (iPSC) line (HEBHMUi002-A) from a healthy female individual and neural differentiation
Summary
Induced pluripotent stem cells (iPSCs) can be used to generate different types of somatic cells in vitro, including neuronal cells. Here, a human iPSC line was generated from the peripheral blood mononuclear cells of a healthy 39-year-old individual. The resulting iPSCs were integration-free, maintained the normal karyotype, expressed pluripotency stem cell markers, and were demonstrated to be capable of differentiating into cells representative of the three embryonic germ layers. Furthermore, we showed that this iPSC line could be differentiated into neural stem cells. Taken together, this generated iPSC line could be useful to test multiple differentiation protocols, and also serve as a control for investigating drug development and disease mechanisms. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ma J, Zhang J, He J, Zhang Z, Li W, Feng B, Guo R, Amponsah AE, Kong D, Liu A, Song Y, Wei L, Cui H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jan;42:101669 |
| PubMed | 31791011 |
| DOI | 10.1016/j.scr.2019.101669 |