Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation


Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous CCR5Δ32 variant to an HIV positive individual has demonstrated efficient long-term control of HIV. We identified three individuals with this homozygous CCR5Δ32 variant, and successfully generated induced pluripotent stem cell (iPSC) lines from their dermal fibroblasts. The iPSCs lines carrying homozygous CCR5Δ32 variant displayed phenotypically normal and the potential to differentiation toward the three germ layers. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Chen G, Jin H, Yu Z, Liu Y, Li Z, Navarengom K, Schwartzbeck R, Dmitrieva N, Cudrici C, Ferrante EA, Biesecker LG, Yang D, Boehm M
Journal Stem cell research
Publication Date 2019 Jul;38:101481
PubMed 31234109
PubMed Central PMC6662727
DOI 10.1016/j.scr.2019.101481

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