I4
TECHe002-A
General
Cell Line |
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| hPSCreg name | TECHe002-A |
| Cite as: | TECHe002-A (RRID:CVCL_C379) |
| Alternative name(s) |
I4
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 4th September 2022 |
| User feedback | |
Provider |
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| Generator | Technion - Israel Institute of Technology (IIT) |
| Derivation country | Israel |
External Databases |
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| Cellosaurus | CVCL_C379 |
| NIHhESC | NIHhESC-13-0205 |
| Wikidata | Q54897063 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternative consent approval number | NIHhESC-13-0205 |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 35
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Other Genotyping (Cell Line) |
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