I4

General

Cell Line

hPSCreg Name
TECHe002-A
Alternative name(s)
I4
Cell line type Human embryonic stem cell (hESC)
Last update 4th September 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Technion - Israel Institute of Technology (IIT)
Derivation country Israel

External Databases

Cellosaurus CVCL_C379
NIHhESC NIHhESC-13-0205
CLO CLO_0100121
Wikidata Q54897063

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternative consent approval number NIHhESC-13-0205
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 35

Other Genotyping (Cell Line)