I4

General#

Cell Line

hPSCreg Name TECHe002-A
Alternative name(s)
I4
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Technion - Israel Institute of Technology (IIT)
Derivation country Israel

External Databases

Cellosaurus CVCL_C379
NIHhESC NIHhESC-13-0205

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)