Lymphoblast-derived integration-free iPSC line AD-TREM2-3 from a 74 year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant


Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, KLF4, LIN28, L-MYC and p53 shRNA. The derived iPSC line - AD-TREM2-3 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.940. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl AR, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Journal Stem cell research
Publication Date 2018 Jul;30:141-144
PubMed 29902745
DOI 10.1016/j.scr.2018.05.018

Research Projects

Cell Lines