Modelling Duchenne muscular dystrophy in vitro with newly generated, blood cell-derived induced pluripotent stem cell line ORIONi003-A


Here, we present newly derived in vitro model for modeling Duchenne muscular dystrophy. Our new cell line was derived by reprogramming of peripheral blood mononuclear cells (isolated from blood from pediatric patient) with Sendai virus encoding Yamanaka factors. Derived iPS cells are capable to differentiate in vitro into three germ layers as verified by immunocytochemistry. When differentiated in special medium, our iPSc formed spontaneously beating cardiomyocytes. As cardiomyopathy is the main clinical complication in patients with Duchenne muscular dystrophy, the cell line bearing the dystrophin gene mutation might be of interest to the research community. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Hajduchova D, Suroviakova S, Mersakova S, Brany D, Zahumenska R, Rehak M, Skovierova H, Nováková S, Nosal V, Marcinek J, Kalman M, Jozef Pec M, Brozmanova M, Melegova J, Juhas S, Juhasova J, Studenovska H, Mitruskova B, Pokusa M, Samec M, Samos M, Nicodemou A, Danisovic L, Dankova Z, Kurca E, Lexova Kolejakova K, Chandoga J, Plank L, Halasova E, Pecova R, Strnadel J
Journal Stem cell research
Publication Date 2023 Sep;71:103187
PubMed 37643496
DOI 10.1016/j.scr.2023.103187

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