Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus


Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of cardiomyocytes, which offers an advantage over the traditional patch-clamp technique. Here, we knocked VSFP into the AAVS1 safe harbor locus of human iPSCs, generating two stable voltage indicator lines - one heterozygous (MRIi003-A-5) and the other homozygous (MRI003-A-6). Both lines can be used for optical membrane potential recordings and provide a powerful platform for a wide range of applications in cardiovascular biomedicine. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang F, Meier AB, Sinnecker D, Engelhardt S, Lipp P, Laugwitz KL, Dorn T, Moretti A
Journal Stem cell research
Publication Date 2022 May;61:102785
PubMed 35421847
DOI 10.1016/j.scr.2022.102785

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