Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus
Summary
Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of cardiomyocytes, which offers an advantage over the traditional patch-clamp technique. Here, we knocked VSFP into the AAVS1 safe harbor locus of human iPSCs, generating two stable voltage indicator lines - one heterozygous (MRIi003-A-5) and the other homozygous (MRI003-A-6). Both lines can be used for optical membrane potential recordings and provide a powerful platform for a wide range of applications in cardiovascular biomedicine. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang F, Meier AB, Sinnecker D, Engelhardt S, Lipp P, Laugwitz KL, Dorn T, Moretti A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 May;61:102785 |
| PubMed | 35421847 |
| DOI | 10.1016/j.scr.2022.102785 |