fWT2, WTF50, fHC2, fCTRL2

General#

Cell Line

hPSCreg Name MUNIi009-A
Alternative name(s)
fWT2, WTF50, fHC2, fCTRL2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th January 2021
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Provider

Generator Masaryk University (MUNI)
Owner Masaryk University (MUNI)
Distributors
Derivation country Czech Republic

External Databases

BioSamples SAMEA7895427
CLO CLO_0103151
Cellosaurus CVCL_A4PH

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity American Indian/Alaska Native

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX[21].arr(1-22,X)x2

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

http://www.personalgenomes.org
Participant #9 (hu034DB1) in the Personal Genome Project

External Databases (Donor)

BioSamples SAMEA7895428

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink https://www.coriell.org/1/About-Us/Contact-Us
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Coriell institute has all documents available
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Masaryk University
Approval number MUNI/G/1131/2017
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Epi5™ Episomal iPSC Reprogramming Kit (cat.:A15960; ThermoFisher)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell line name GM23251
Source cell type
Fibroblasts
Source cell origin
Skin of arm
Synonyms
  • Arm skin
Age of donor (at collection) 50-54
Source cell line vendor Coriell Institute for Medical Research

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection PCR detection of Epstein-Barr virus nuclear antigen 1 (EBNA1) gene and oriP element of Epi5™ Episomal iPSC Reprogramming Kit
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones The main selection criteria were morphology, all tested clones have high expression of pluripotent markers
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Supplements
ZellShield 1X %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-81
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
AFP
Yes
Morphology
fCTRL2 D15
Protocol or reference
Cultivation protocols for PSCs.pdf
Embryoid bodies - polyhema 96W
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
fCTRL2 D15
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
MAP2
Yes
Morphology
fCTRL2 D15
Protocol or reference
Cultivation protocols for PSCs.pdf
Embryoid bodies - polyhema 96W

Microbiology / Virus Screening

Mycoplasma Positive

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: p23
Karyotyping method: G-Banding

Other Genotyping (Cell Line)