INNDSUi002-A

21-013

General

Cell Line

hPSCreg name INNDSUi002-A
Cite as:
INNDSUi002-A (RRID:CVCL_D0G3)
Alternative name(s)
21-013
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 21st December 2022
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Provider

Generator Institute of Neuromuscular and Neurodegenerative Diseases, Shandong University (INNDSU)

External Databases

BioSamples SAMEA13061467
Cellosaurus CVCL_D0G3
Wikidata Q123032706

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Multiple Acyl-CoA Dehydrogenase Deficiency
  • Glutaric Acidemia Type 2
  • Multiple Acyl Coenzyme A Dehydrogenase Deficiency
  • Glutaric Aciduria, Type 2
show more synonyms

External Databases (Donor)

BioSamples SAMEA13061468

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. fuchen.liu@email.sdu.edu.cn
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information fuchen.liu@email.sdu.edu.cn
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Qilu Hospital of Shandong University, Jinan
Approval number 2020066
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Induced pluripotent stem cells (iPS cells or iPSCs) are a type of pluripotent stem cell artificially derived from a non-pluripotent cell. Various methods exist to revert cells to pluripotency such as reprogramming mediated through a mature metaphase II oocyte as in somatic cell nuclear transfer.; Submitted by Nancy Mah
Synonyms
  • iPSC
  • iPS cell

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium:
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
HNF3B
Yes
Alpha-fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
alpha smooth muscle
Yes
brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
TUBB3
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY, Resolution 400-450

Other Genotyping (Cell Line)