Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Summary

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained fullpluripotency, normal karyotype and spontaneousdifferentiation capability. This cell line provides a suitable model for investigating the physiopathologyof PITX2 mutation in atrial fibrillation. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Mun D, Kang JY, Chun Y, Park DS, Kim H, Yun N, Lee SH, Joung B
Journal Stem cell research
Publication Date 2022 Oct 13;65:102940
PubMed 36260954
DOI 10.1016/j.scr.2022.102940

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