Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system
Summary
PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained fullpluripotency, normal karyotype and spontaneousdifferentiation capability. This cell line provides a suitable model for investigating the physiopathologyof PITX2 mutation in atrial fibrillation. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Mun D, Kang JY, Chun Y, Park DS, Kim H, Yun N, Lee SH, Joung B |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Dec;65:102940 |
| PubMed | 36260954 |
| DOI | 10.1016/j.scr.2022.102940 |