Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus
Summary
We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1-60, NANOG and SSEA4) and in vitro differentiation into three germ layers. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Orsi A, Serpieri V, Mazzotta C, Ramusino MC, Rossi E, Cerri S, Valente EM |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103332 |
| PubMed | 38354648 |
| PubMed Central | PMC10983824 |
| DOI | 10.1016/j.scr.2024.103332 |