Generation of a human induced pluripotent stem cell line (UNIFEi001-A) from a patient with Spinocerebellar ataxia type 1 (SCA1)
Summary
Spinocerebellar ataxia type 1 (SCA1) is one of six SCAs related to the expansion of a CAG repeat, encoding glutamine. Induced pluripotent stem cells were generated by reprogramming fibroblasts isolated from a patient affected by SCA1, through the transduction of three viral vectors derived from a non-integrative virus. UNIFEi001-A cell line presents an ESC-like morphology, is vector-free, expresses pluripotency markers, presents a normal karyotype and can differentiate into the three germ layers. These cells can be further differentiated into neural stem cells and neurons to serve as a cellular model. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Pappadà M, Melija S, Facchini M, Martino M, Minarini S, Caproni A, Nordi C, Rimessi P, Selvatici R, Marconi P |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec 22;83:103637 |
| PubMed | 39892158 |
| DOI | 10.1016/j.scr.2024.103637 |