General

Cell Line
hPSCreg name	VIACe001-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VIACe001-A-1 (RRID:CVCL_9728)
Alternative name(s)	BG01V/hOG
Cell line type	Human embryonic stem cell (hESC)
Similar lines	VIACe001-A (hESBGN-01 (BG01)) VIACe001-A-2 (BG01V)
Last update	28th July 2017
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Provider
Generator	Novocell, Inc. (VIAC)
Derivation country	United States
External Databases
Cellosaurus	CVCL_9728
Wikidata	Q54796221
General Information
Publications	Freitas ER et al. Feeder-free culture of human embryonic stem cell line BG01V/hOG using magnetic field-magnetic nanoparticles system. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2013 Feb;67(1):17-21.
* Is the cell line readily obtainable for third parties?	Yes
Subclone of	VIACe001-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed.

Ethics

Also have a look at the ethics information for the parental line VIACe001-A .

Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line VIACe001-A.

Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
Passage method	Enzymatically Collagenase
CO2 Concentration	5 %
Medium	Other medium: Base medium: DMEM/F12 with Glutamax Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit glutamax bFGF 374_102_invitrogen_BG01Vhog.pdf

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
hOct4-GFP	Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease

Emerald Green Fluorescent Protein (target) Type of modification Transgene expression Chromosome location NA Free text This is a commercially available embryonic stem cell line (Invitrogen Cat No. R7799-105). Delivery method An integration vector containing an EmGFP gene directed by the human Oct4 promoter and followed by the HSV-TK polyadenylation signal was stably integrated into the genome of BG01V cells.