General

iPSC Line
hPSCreg name	VIACe001-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VIACe001-A-1 (RRID:CVCL_9728)
Alternative name(s)	BG01V/hOG
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	VIACe001-A (hESBGN-01 (BG01)) VIACe001-A-2 (BG01V)
Last update	28th July 2017
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	Novocell, Inc. (VIAC)
Derivation country	United States
External iPSC Databases
Cellosaurus	CVCL_9728
Wikidata	Q54796221
General iPSC Information
Publications	Freitas ER et al. Feeder-free culture of human embryonic stem cell line BG01V/hOG using magnetic field-magnetic nanoparticles system. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2013 Feb;67(1):17-21.
* Is the cell line readily obtainable for third parties?	Yes
Subclone of	VIACe001-A

iPSC Genetic Modification

Genetic modifications not related to a disease

Emerald Green Fluorescent Protein (target) Type of iPSC Modification Transgene expression Chromosome location NA Free text This is a commercially available embryonic stem cell line (Invitrogen Cat No. R7799-105). Delivery method An integration vector containing an EmGFP gene directed by the human Oct4 promoter and followed by the HSV-TK polyadenylation signal was stably integrated into the genome of BG01V cells.

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed.

Ethics

Also have a look at the ethics information for the parental line VIACe001-A .

Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line VIACe001-A.

iPSC Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
Passage method	Enzymatically Collagenase
CO2 Concentration	5 %
Medium	Other medium: Base medium: DMEM/F12 with Glutamax Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit glutamax bFGF 374_102_invitrogen_BG01Vhog.pdf

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
hOct4-GFP	Yes

Genotyping

Karyotyping (iPSC Line)

Other Genotyping (iPSC Line)