VIACe001-A-1

BG01V/hOG

General#

Cell Line

hPSCreg Name VIACe001-A-1
Alternative name(s)
BG01V/hOG
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Novocell, Inc. (VIAC)
Derivation country United States

External Databases

Cellosaurus CVCL_9728

General Information

Publications
Is the cell line available in principle?
Yes
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2001-03-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
hOct4-GFP
Yes

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Emerald Green Fluorescent Protein (target)
Transgene expression
NA
This is a commercially available embryonic stem cell line (Invitrogen Cat No. R7799-105).
An integration vector containing an EmGFP gene directed by the human Oct4 promoter and followed by the HSV-TK polyadenylation signal was stably integrated into the genome of BG01V cells.