BG01V
VIACe001-A-2
General
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed. |
Ethics
Also have a look at the ethics information for the parental line
VIACe001-A
.
| Alternatives to consent |
hESC Derivation
|
The source cell information can be found in the parental cell line
VIACe001-A.
|
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
|||||||||
| Passage method |
Enzymatically
Collagenase
|
|||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: DMEM F/12
Main protein source: Fetal bovine serum Serum concentration: 15 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
|
|
||
| SSEA-3 |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
|
|||
| TRA 1-60 |
Yes |
|
|
|||
| TRA 1-81 |
Yes |
|
|
|||
| ABCG2 |
Yes |
|
||||
| DNMT3B |
Yes |
|
||||
| DPPA4 |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| TDGF1 |
Yes |
|
||||
| TERT |
Yes |
|
||||
| UTF1 |
Yes |
|
||||
| SSEA-1 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
49XXY,+12,+17
Passage number: 45
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.