HS401

General

Cell Line

hPSCreg name KIe019-A
Cite as:
KIe019-A (RRID:CVCL_C212)
Alternative name(s)
HS401
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 8th May 2022
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Provider

Generator Karolinska Institutet (KI)
Derivation country Sweden

External Databases

Cellosaurus CVCL_C212
NIHhESC NIHhESC-13-0202
BioSamples SAMEA6407453
Wikidata Q54896320

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA6407456

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information ganesh.acharya@ki.se
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-13-0202
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regional ethical committee
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Regional Ethics Committee, Stockholm, Sweden
Approval number 454/02

hESC Derivation

Date of derivation 2005-05-12
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: %
Supplements
FGF-2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 12

Other Genotyping (Cell Line)