Man-16

General#

Cell Line

hPSCreg Name CMFTe004-A
Alternative name(s)
Man-16
Cell line type Human embryonic stem cell (hESC)
Last update 14th November 2017

Provider

Generator Central Manchester and Manchester Children's University Hospitals NHS (CMFT)
Owner Central Manchester NHS Foundation Trust (CMFT)
Distributors National Institute for Biological Standards and Control - UK Stem Cell Bank (USCB)
Derivation country United Kingdom

External Databases

BioSamples SAMEA104615891

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for clinical research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal Male 46 XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

External Databases (Donor)

BioSamples SAMEA104619381

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2012-09-18
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating CELLstart
Feeder cells Human Dermal Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 2
Supplements
Penicillin/streptomycin 1 %
L-Glutamine 2 mM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unkown

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
GATA Binding Protein 6
Yes
FOXA2
Yes
Sebaceous Gland
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Actin, Alpha 2, Smooth Muscle, Aorta
Yes
Vimentin
Yes
Smooth Muscle Tissue
Ont Id: UBERON_0001135
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Tubulin Beta 3 Class III
Yes
Neurofilament, light polypeptide
Yes
Skin Tissue
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal Male 46 XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

Genetic Modification#

Genetic Modifications (other than for reprogramming)