Man-16

General

Cell Line

hPSCreg Name
CMFTe004-A
Alternative name(s)
Man-16
Cell line type Human embryonic stem cell (hESC)
Last update 31st January 2022
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Provider

Generator Central Manchester and Manchester Children's University Hospitals NHS (CMFT)
Owner Central Manchester NHS Foundation Trust (CMFT)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA104615891
Cellosaurus CVCL_BW70
CLO CLO_0101455
Wikidata Q54903992

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal Male 46 XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

External Databases (Donor)

BioSamples SAMEA104619381

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HTA, HFEA, and UK Stem Cell Bank
Approval number HTA Licence number: 22627, HFEA Licence number: R0171-4-b. UKSCB approval number: SCSC14-25

hESC Derivation

Date of derivation 2012-09-18
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating CELLstart
Feeder cells Human Dermal Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 2
Supplements
Penicillin/streptomycin 1 %
L-Glutamine 2 mM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
GATA Binding Protein 6
Yes
FOXA2
Yes
Sebaceous Gland
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Actin, Alpha 2, Smooth Muscle, Aorta
Yes
Vimentin
Yes
Smooth Muscle Tissue
Ont Id: UBERON_0001135
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Tubulin Beta 3 Class III
Yes
Neurofilament, light polypeptide
Yes
Skin Tissue
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal Male 46 XY
Passage number: 16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH