RC-16, RC16

General#

Cell Line

hPSCreg Name RCe020-A
Alternative name(s)
RC-16, RC16
Cell line type Human embryonic stem cell (hESC)
Last update 11th September 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L205

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2010-10-11
Embryo stage Zygote
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Manual Removal of ZP
Cell isolation Trophoectoderm and ICM isolated
Cell seeding Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Morphology pictures
Morphology of colonies of passages 6 and 17
Differentiation Potency
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX, +14, i(20)(q10)

Other Genotyping (Cell Line)