Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
Summary
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. Copyright © 2015 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.
| Authors | Questa M, Romorini L, Blüguermann C, Solari CM, Neiman G, Luzzani C, Scassa MÉ, Sevlever GE, Guberman AS, Miriuka SG |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Mar;16(2):300-3 |
| PubMed | 27345989 |
| DOI | 10.1016/j.scr.2015.12.026 |