Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient


Fibroblasts were isolated from skin biopsies from two patients with bipolar I disorder. One patient was a 26 year old female carrying a risk haplotype in the DGKH (diacylglycerol kinase eta) gene and the other was a non-carrier 27 year old male. Patient fibroblasts were reprogrammed into human induced pluripotent stem cells (hiPSCs) by using a Sendai virus vector. DGKH-risk haplotype and non-risk haplotype hiPSCs showed expression of pluripotency markers and were able to differentiate into cells of the three germ layers. These cell models are useful to investigate the role of risk gene variants in bipolar disorder. Resource table. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Palladino VS, Subrata NOC, Geburtig-Chiocchetti A, McNeill R, Hoffmann P, Reif A, Kittel-Schneider S
Journal Stem cell research
Publication Date 2018 Oct;32:104-109
PubMed 30266033
DOI 10.1016/j.scr.2018.09.008

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