AR1034

General

Cell Line

hPSCreg name KGUi002-A
Cite as:
KGUi002-A (RRID:CVCL_VR58)
Alternative name(s)
AR1034
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
KGUi001-A
(AR1023)
Donor's gene variants:
DGKH
Donor diseases:
obsolete_bipolar disorder
STBCi145-A
(SFC125-03-01)
Donor diseases:
bipolar disorder
STBCi146-A
(SFC127-03-01)
Donor diseases:
bipolar disorder
STBCi150-A
(SFC133-03-01)
Donor diseases:
bipolar disorder
STBCi152-A
(SFC146-03-01)
Donor diseases:
bipolar disorder
STBCi154-A
(SFC148-03-01)
Donor diseases:
bipolar disorder
STBCi155-A
(SFC149-03-01)
Donor diseases:
bipolar disorder
STBCi170-A
(SFC190-03-01)
Donor diseases:
bipolar disorder
STBCi068-A
(SFC035-03-01)
Donor diseases:
bipolar disorder
STBCi068-B
(SFC035-03-02)
Donor diseases:
bipolar disorder
STBCi068-C
(SFC035-03-03)
Donor diseases:
bipolar disorder
STBCi210-A
(SFC305-03-01)
Donor diseases:
bipolar disorder
STBCi211-A
(SFC306-04-01)
Donor diseases:
bipolar disorder
STBCi076-A
(SFC134-03-01)
Donor diseases:
bipolar disorder
STBCi111-A
(SFC131-03-01)
Donor diseases:
bipolar disorder
STBCi114-A
(SFC034-03-01)
Donor diseases:
bipolar disorder
STBCi130-A
(SFC172-03-01)
Donor diseases:
bipolar disorder
STBCi091-A
(SFC126-03-01)
Donor diseases:
bipolar disorder
STBCi091-B
(SFC126-03-02)
Donor diseases:
bipolar disorder
STBCi091-C
(SFC126-03-03)
Donor diseases:
bipolar disorder
STBCi136-A
(SFC036-03-01)
Donor diseases:
bipolar disorder
STBCi249-A
(SFC171-03-01)
Donor diseases:
bipolar disorder
STBCi147-A
(SFC128-03-01)
Donor diseases:
bipolar disorder
STBCi148-A
(SFC130-03-01)
Donor diseases:
bipolar disorder
STBCi149-A
(SFC132-03-01)
Donor diseases:
bipolar disorder
STBCi151-A
(SFC145-03-01)
Donor diseases:
bipolar disorder
STBCi153-A
(SFC147-03-01)
Donor diseases:
bipolar disorder
STBCi167-A
(SFC174-03-01)
Donor diseases:
bipolar disorder
STBCi168-A
(SFC176-03-01)
Donor diseases:
bipolar disorder
STBCi169-A
(SFC179-03-01)
Donor diseases:
bipolar disorder
STBCi172-A
(SFC192-03-01)
Donor diseases:
bipolar disorder
STBCi173-A
(SFC193-03-01)
Donor diseases:
bipolar disorder
STBCi174-A
(SFC194-03-01)
Donor diseases:
bipolar disorder
STBCi208-A
(SFC303-04-01)
Donor diseases:
bipolar disorder
STBCi209-A
(SFC304-04-01)
Donor diseases:
bipolar disorder
STBCi075-A
(SFC129-03-02)
Donor diseases:
bipolar disorder
STBCi075-B
(SFC129-03-03)
Donor diseases:
bipolar disorder
STBCi212-A
(SFC312-03-01)
Donor diseases:
bipolar disorder
STBCi075-C
(SFC129-03-01)
Donor diseases:
bipolar disorder
STBCi081-A
(SFC170-03-01)
Donor diseases:
bipolar disorder
STBCi081-B
(SFC170-03-03)
Donor diseases:
bipolar disorder
STBCi112-A
(SFC177-03-01)
Donor diseases:
bipolar disorder
STBCi113-A
(SFC178-03-01)
Donor diseases:
bipolar disorder
STBCi118-A
(SFC037-03-01)
Donor diseases:
bipolar disorder
STBCi125-A
(SFC175-03-01)
Donor diseases:
bipolar disorder
STBCi081-C
(SFC170-03-02)
Donor diseases:
bipolar disorder
STBCi135-A
(SFC033-03-01)
Donor diseases:
bipolar disorder
EDi011-B
(RCi140)
Donor diseases:
bipolar disorder
EDi011-C
(RCi141)
Donor diseases:
bipolar disorder
EDi011-A
(RCi139)
Donor diseases:
bipolar disorder
Last update 18th October 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Department of Psychiatry, Psychotherapy and Psychosomatic Medicine, University Hospital, Goethe University of Frankfurt/Main (KGU)
Owner Department of Psychiatry, Psychotherapy and Psychosomatic Medicine, University Hospital, Goethe University of Frankfurt/Main (KGU)
Derivation country Germany

External Databases

BioSamples SAMEA4861683
Cellosaurus CVCL_VR58
Wikidata Q94338896

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
Depressed Bipolar I
Synonyms
  • Psychoses, Manic-Depressive
  • Bipolar affective disorder, current episode depression (disorder)
  • Manic bipolar I disorder
  • Manic-depressive psychosis
  • mixed bipolar affective disorder, NOS (disorder)
  • Disorder, Bipolar
  • Manic bipolar I disorder (disorder)
  • Manias
  • Bipolar Disorders
  • Affective Bipolar Psychosis
  • Psychosis, Bipolar Affective
  • Psychosis, Manic-Depressive
  • Manic Depressive Psychosis
  • MANIC DIS
  • BIPOLAR DIS
  • Manic Disorders
  • Unspecified bipolar affective disorder, NOS (disorder)
  • Bipolar affective disorder
  • Manic States
  • Unspecified bipolar affective disorder
  • State, Manic
  • Psychoses, Manic Depressive
  • MANIC DEPRESSIVE ILLNESS
  • Mania
  • bipolar disorder manic phase
  • Unspecified bipolar affective disorder, NOS
  • Psychoses, Bipolar Affective
  • Unspecified bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder, unspecified
  • Psychosis, Manic Depressive
  • Bipolar affective disorder , current episode mixed (disorder)
  • Disorder, Manic
  • Manic-Depressive Psychoses
  • Manic Disorder
  • States, Manic
  • mixed bipolar disorder
  • [X]Bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder (disorder)
  • Bipolar disorder, unspecified
  • Bipolar Affective Psychosis
  • Manic-depressive syndrome NOS
  • Manic Bipolar Affective disorder
  • Manic State
  • Bipolar affective disorder, manic, unspecified degree
  • Bipolar disorder (disorder)
  • mixed bipolar affective disorder (disorder)
  • Affective Psychosis, Bipolar
  • [X]Bipolar affective disorder, unspecified
  • bipolar disease
  • Bipolar affective disorder, mixed, unspecified degree
  • MDI - Manic-depressive illness
  • Manic-depressive illness
  • Bipolar disorder, NOS
  • BIPOLAR DISORDER NOS
  • mixed bipolar I disorder (disorder)
  • Depression, Bipolar
  • Depressive-manic psych.
  • Manic Depressive disorder
  • Bipolar Depression
  • Manic-Depression
show more synonyms
Genetic variants
DGKH (target)
13q14.11
Heterozygous
Disease associated phenotypes no phenotypes
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
CGH Array data available on request
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA4861684

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: data is stored in our database and can given in pseudonymised form
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: data is stored in our database and can given in pseudonymised form
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics committee University of Würzburg, 10.06.2011
Approval number 96/11
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics committee University of Frankfurt, 04.3.2015
Approval number 425/14
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? CytoTune-Ips 2.0 Sendai Reprogramming Kit (Invitrogen)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Passage number reprogrammed 5

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Early clone selection was performed by Alkaline Phosphatase Live Stain (Life Technologies) and a fluorescence microscope equipped with a FITC filter (Leica). Fluorescent-labelled colonies were manually picked and transferred onto new cultureware for single clone expansion.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells mouse embryonic fibroblast
Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ 2
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Embryoid body assay, pluripotency markers verified by PCR and immunostaining
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
CGH Array was conducted of the donor blood, fibros and hiPS; data are available on request
Passage number: 12
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes