AR1023

General#

Cell Line

hPSCreg Name KGUi001-A
Alternative name(s)
AR1023
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th May 2018
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Provider

Generator Department of Psychiatry, Psychotherapy and Psychosomatic Medicine, University Hospital, Goethe University of Frankfurt/Main (KGU)
Owner Department of Psychiatry, Psychotherapy and Psychosomatic Medicine, University Hospital, Goethe University of Frankfurt/Main (KGU)

External Databases

BioSamples SAMEA4861682
Cellosaurus CVCL_VR57

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
bipolar disorder
The donor is a carrier of a disease-associated mutation and affected.
Stage
Depressed Bipolar I
Synonyms
  • Psychoses, Manic-Depressive
  • Bipolar affective disorder, current episode depression (disorder)
  • Manic bipolar I disorder
  • Manic-depressive psychosis
  • mixed bipolar affective disorder, NOS (disorder)
  • Disorder, Bipolar
  • Manic bipolar I disorder (disorder)
  • Manias
  • Bipolar Disorders
  • Affective Bipolar Psychosis
  • Psychosis, Bipolar Affective
  • Psychosis, Manic-Depressive
  • Manic Depressive Psychosis
  • MANIC DIS
  • Bipolar Depression
  • BIPOLAR DIS
  • Manic Disorders
  • Unspecified bipolar affective disorder, NOS (disorder)
  • Bipolar affective disorder
  • Manic States
  • Manic Depressive disorder
  • Unspecified bipolar affective disorder
  • State, Manic
  • Psychoses, Manic Depressive
  • MANIC DEPRESSIVE ILLNESS
  • Mania
  • bipolar disorder manic phase
  • Unspecified bipolar affective disorder, NOS
  • Psychoses, Bipolar Affective
  • Unspecified bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder, unspecified
  • Psychosis, Manic Depressive
  • Bipolar affective disorder , current episode mixed (disorder)
  • Disorder, Manic
  • Manic-Depressive Psychoses
  • Manic Disorder
  • States, Manic
  • mixed bipolar disorder
  • [X]Bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder (disorder)
  • Bipolar disorder, unspecified
  • Bipolar Affective Psychosis
  • Manic-depressive syndrome NOS
  • Manic Bipolar Affective disorder
  • Manic State
  • Bipolar affective disorder, manic, unspecified degree
  • Bipolar disorder (disorder)
  • mixed bipolar affective disorder (disorder)
  • Affective Psychosis, Bipolar
  • [X]Bipolar affective disorder, unspecified
  • bipolar disease
  • Bipolar affective disorder, mixed, unspecified degree
  • MDI - Manic-depressive illness
  • Manic-depressive illness
  • Bipolar disorder, NOS
  • BIPOLAR DISORDER NOS
  • mixed bipolar I disorder (disorder)
  • Depression, Bipolar
  • Depressive-manic psych.
  • Manic-Depression
show more synonyms
Genetic variants
DGKH (target)
13q14.11
Heterozygous
DGKH gene variants have been associated with bipolar disorder.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
molecular karyotping
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA4861682

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Passage number reprogrammed 5

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Early clone selection was performed by Alkaline Phosphatase Live Stain (Life Technologies) and a fluorescence microscope equipped with a FITC filter (Leica). Fluorescent-labelled colonies were manually picked and transferred onto new cultureware for single clone expansion.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells mouse embryonic fibroblast
Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
CGH Array was conducted; data are available on request
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH