Generation of CSi001-A, a transgene-free, induced pluripotent stem cell line derived from a Parkinson Disease (PD) patient


Peripheral blood mononuclear cells (PBMC) were collected from a 70-year old Parkinson Disease patient. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated directly toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhao Z, Ji S, Shi Z, Liu H
Journal Stem cell research
Publication Date 2018 Dec;33:1-5
PubMed 30292881
DOI 10.1016/j.scr.2018.09.020

Research Projects

Cell Lines