Unveiling tissue-specific transcriptional adaptations in iPSC-derived fibroblasts via co-culture systems
Summary
These findings demonstrate that iFBs can adopt tissue-specific transcriptional profiles, supporting their potential for modelling fibrotic microenvironments in 3D in vitro systems. However, the partial and transient nature of these adaptations underscores the need to validate whether transcriptional changes translate to functional fibroblast behaviours, such as ECM dysregulation or aberrant TGF-β signalling, in complex tissue models. Optimising co-culture conditions to stabilise these phenotypes will be critical for leveraging iFBs in fibrosis research, drug screening, and personalised disease modelling. © 2025. The Author(s).
| Authors | Azad AJ, Bentivogli A, Germar H, Wörz D, Lizunova E, Cumberland MJ, Weiner J, Hedtrich S |
|---|---|
| Journal | Stem cell research & therapy |
| Publication Date | 2025 Jul 30;16(1):413 |
| PubMed | 40739279 |
| PubMed Central | PMC12312452 |
| DOI | 10.1186/s13287-025-04537-6 |