TC-113

General#

Cell Line

hPSCreg Name RUCDRi002-A
Alternative name(s)
TC-113
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th September 2018
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Provider

Generator RUCDR Infinite Biologics (RUCDR)
Owner NIH - National Institute of Neurological Disorders and Stroke (NINDS)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA4933040

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4938814

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name Umbillical Cord Blood Cell (CD34+)
Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
umbilical cord
The connecting cord from the developing embryo to the placenta.
Synonyms
  • chorda umbilicalis
  • funiculus umbilicalis

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating L7 Matrix (Lonza)
Feeder cells
No
Passage method L7 hPSC Passaging Solution (Lonza)
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Marker Present Absent
mCpG
OCT4 X
Pluripotency Score Novelty Score
44.56 1.78
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
Neuron
Ont Id: CL_0000540

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)