Protocol to develop force-generating human skeletal muscle organoids


Force generation is an essential property of skeletal muscle models in vitro. We describe a versatile 1-step procedure to direct undifferentiated human pluripotent stem cells (PSCs) into contractile skeletal muscle organoids (SMOs). Our protocol provides detailed steps for 3D casting of PSCs using either collagen-I/Matrigel- or fibrin/Geltrex-based hydrogels, SMO differentiation, and application of different culture platforms for mechanical loading and contractility analysis. The SMO model may be particularly useful to study human muscle development and developmental skeletal muscle disorders in vitro. For complete details on the use and execution of this protocol, please refer to Shahriyari et al.1. Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Authors Shahriyari M, Rinn M, Hofemeier AD, Babych A, Zimmermann WH, Tiburcy M
Journal STAR protocols
Publication Date 2024 Mar 15;5(1):102794
PubMed 38133957
PubMed Central PMC10776636
DOI 10.1016/j.xpro.2023.102794

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