Generation of induced pluripotent stem cells from peripheral blood CD34+ hematopoietic progenitors of a 31year old healthy woman
Summary
The MUi019-A human induced pluripotent stem cell line was generated from peripheral blood CD34+ hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019-A to model disease while the cell type affected by the disease is inaccessible. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Tangprasittipap A, Jittorntrum B, Wongkummool W, Kitiyanant N, Tubsuwan A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Apr;20:91-93 |
| PubMed | 28395748 |
| DOI | 10.1016/j.scr.2017.02.013 |