Royan H1
RIe001-A
General
Cell Line |
|
| hPSCreg name | RIe001-A |
| Cite as: | RIe001-A (RRID:CVCL_9381) |
| Alternative name(s) |
Royan H1
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
Royan Institute (RI)
Contact:
Embryonic Stem Cell Biology |
| Derivation country | Iran |
External Databases |
|
| Cellosaurus | CVCL_9381 |
| Wikidata | Q54950982 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | None |
| Cell seeding | Embryo |
Culture Conditions
| Surface coating | Gelatin | ||||||||||||||||||||||||
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
||||||||||||||||||||||||
| Passage method | Mechanically | ||||||||||||||||||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 30
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.