Generation of green fluorescent protein reporter knock-in iPSC line at the 3'UTR region of the KLOTHO locus
Summary
We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3'-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids. Copyright © 2024. Published by Elsevier B.V.
| Authors | Lim SW, Lee KI, Cui S, Fang X, Shin YJ, Lee H, Lee JY, Chung BH, Yang CW |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Oct;80:103499 |
| PubMed | 39111000 |
| DOI | 10.1016/j.scr.2024.103499 |