DMD02

General#

Cell Line

hPSCreg Name MUNIi001-A
Alternative name(s)
DMD02
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th February 2019
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Provider

Generator Masaryk University (MUNI)
Owner Petr Dvorak's Laboratory
Distributors
Derivation country Czech Republic

External Databases

Cellosaurus CVCL_9S28
BioSamples SAMEA5466088

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Duchenne muscular dystrophy
The donor is affected.
Synonyms
  • Muscular dystrophy, Duchenne
Non-disease associated phenotypes
  • Deletion of exon 45-50 resulting in frameshift by MLPA
  • Severe neurological diagnosis
Family history mother mutation carrier, no previouse family histry
Is the medical history available upon request? rapid progression, mild cardiac phenotype
Is clinical information available? hypotension, limb swelling

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Donor Relations

All cell lines of this donor's relatives
Has mother:

External Databases (Donor)

BioSamples SAMEA5466090

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Alternatives to consent
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Policy for access to genetic information Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: Masaryk University, Pediatric Neurology Clinic
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee, Medical faculty, Masaryk University
Approval number 13/2012
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee, Medical faculty, Masaryk University
Approval number 13/2012
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Life Technologies
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell type
Skin of arm
Synonyms
  • Arm skin
Source cell origin
skin of leg
A zone of skin that is part of a leg [Automatically generated definition].
Synonyms
  • leg zone of skin
  • zone of skin of leg
  • leg skin
Age of donor (at collection) 10-14
Collected in 2013
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Selection criteria for clones morphology, alcaline phophatase, SSEA, Nanog
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblast
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Dulbecco`s modified Eagle medium (DMEM)/F12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
human FGF2 4 ng/ml
MEM Non-essential Amino Acids 1 %
penicillin-streptomycin 0,5 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Cardiac Myocyte
Ont Id: FMA_14067
In vitro spontaneous differentiation
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)