FJMUNi001-A
General
Cell Line |
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hPSCreg Name | FJMUNi001-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 25th February 2022 |
User feedback | |
Provider |
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Generator | Department of Neurology, Fujian Institute of Neurology, the First Affiliated Hospital, Fujian Medical University (FJMUN) |
Owner | Department of Neurology, Fujian Institute of Neurology, the First Affiliated Hospital, Fujian Medical University (FJMUN) |
Distributors | |
Derivation country | China |
External Databases |
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BioSamples | SAMEA12095262 |
CLO | CLO_0103698 |
Cellosaurus | CVCL_C0FR |
Wikidata | Q112929563 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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External Databases (Donor) |
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BioSamples | SAMEA12095263 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Provide contact information of the holder of the original Donor Information Sheet: | Tel 0591-87982772 |
Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | No information |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | the First Affiliated Hospital, Fujian Medical University |
Approval number | 闽医大附一伦理医研【2018】019号 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type |
FibroblastSynonyms
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
PCR, Sequencing
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
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CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: ncTarget hiPSC Medium
Main protein source: Serum concentration: % |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
karyogram
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Other Genotyping (Cell Line) |
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