DMD04

General#

Cell Line

hPSCreg Name MUNIi004-A
Alternative name(s)
DMD04
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th August 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Masaryk University (MUNI)
Owner Petr Dvorak's Laboratory
Distributors
Derivation country Czech Republic

External Databases

Cellosaurus CVCL_RL58

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 15-19
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Duchenne muscular dystrophy
point mutation in exon 21, 2623C>G
The donor is affected.
Stage
immobile, non-sustained respirator need, cardiac manifestation
Synonyms
  • Muscular dystrophy, Duchenne
Family history no related to case
Is the medical history available upon request? rapid progression, mild cardiac phenotype
Is clinical information available? hypotension, limb swelling

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: Masaryk University, Pediatric Neurology Clinic
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee, Medical faculty, Masaryk University
Approval number 13/2012
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee, Medical faculty, Masaryk University
Approval number 13/2012
Do you have obligations to third parties in regard to the use of the cell line? No
Please describe:
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Further constraints on use
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Life Technologies
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No
Constraints for use or distribution

hIPSC Derivation#

General

Source cell type
Skin of arm
Synonyms
  • Arm skin
Source cell origin
skin of abdomen
A zone of skin that is part of an abdomen [Automatically generated definition].
Synonyms
  • abdomen zone of skin
  • skin of abdomen proper
  • abdominal skin
  • abdomen skin
  • zone of skin of abdomen
show more synonyms
Age of donor (at collection) 15-19
Collected in 2015
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Selection criteria for clones morphology, alcaline phophatase, SSEA, Oct4, Nanog
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Dulbecco`s modified Eagle medium (DMEM)/F12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
MEM Non-essential Amino Acids 1 %
penicillin-streptomycin 0,5 %
human FGF2 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
SSEA-4
Yes
Morphology pictures

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)