DMD1_4

General

Cell Line

hPSCreg name ICGi002-B
Cite as:
ICGi002-B (RRID:CVCL_YK91)
Alternative name(s)
DMD1_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ICGi002-C
(DMD1_11)
Donor diseases:
Duchenne Muscular Dystrophy
ICGi002-A
(DMD1_1)
Donor diseases:
Duchenne Muscular Dystrophy
CENSOi003-B
(FB79R2c6, CENSOi258)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi005-A
(FB76R2c5, CENSOi245)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi007-A
(FB75R2c5, CENSOi255)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
MRIi027-A
(DMD 01)
Donor diseases:
Duchenne muscular dystrophy
CENSOi001-B
(FB78R2c2, CENSOi249)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi002-B
(FB74R2c4, CENSOi261)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
RGIe086-A
(SI-180)
Donor diseases:
Duchenne muscular dystrophy
Last update 29th November 2019
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Provider

Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA6351049
Cellosaurus CVCL_YK91
Wikidata Q94313401

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Duchenne Muscular Dystrophy
  • Duchenne

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6098719

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? St. Petersburg State Pediatric Medical University ethics committee
Approval number 09/05
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? St. Petersburg State Pediatric Medical University ethics committee
Approval number 09/05
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name DMD1
Derived from same source line (potentially other lot and donor, see below):
Source cell type
A cell having only one nucleus, especially: MONOCYTE.
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms
Collected in 2018

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
TrypLE
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
NEAA 0.1 mM
L-glutamine 2 mM
2-Mercaptoethanol 0.25 mM
penicillin 100 U/ml
streptomycin 100 µg/ml
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
LIN28A
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
NEFH
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 18
Karyotyping method: G-Banding

Other Genotyping (Cell Line)